Hotspot

rpoB-trnC-GCA

LSC · 27154-28570 · 1417 bp

Back to Poilannammia

Pi 0.00918

MarkerSeek score 55.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1417
variable_sites: 13
pi: 0.00918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCACTTCCATCCACCAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	1499-1518 bp	1.0	72.0
rpoB-trnC-GCA_p2	TTCCCTCACTTCCATCCACC	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.0	58.1	1504-1523 bp	1.0	72.0
rpoB-trnC-GCA_p3	GACATTCCCTCACTTCCATCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.1	58.1	1508-1527 bp	1.0	71.5
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1506-1525 bp	1.0	71.2
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1505-1524 bp	1.0	71.2