Hotspot

trnR-UCU-atpA

LSC · 9883-10191 · 309 bp

Back to Poilannammia

Pi 0.0194

MarkerSeek score 63.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 6
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AACACCAAACCAGAAGCCAA	CATTCACCGAGGAAGCAGAA	45.0	50.0	58.1	57.9	495-547 bp	1.0	79.0
trnR-UCU-atpA_p2	ACACCAAACCAGAAGCCAAA	CATTCACCGAGGAAGCAGAA	45.0	50.0	58.1	57.9	494-546 bp	1.0	79.0
trnR-UCU-atpA_p3	AACACCAAACCAGAAGCCAA	AAGACATTCACCGAGGAAGC	45.0	50.0	58.1	57.9	499-551 bp	1.0	79.0
trnR-UCU-atpA_p4	ACACCAAACCAGAAGCCAAA	AAGACATTCACCGAGGAAGC	45.0	50.0	58.1	57.9	498-550 bp	1.0	79.0
trnR-UCU-atpA_p5	AGAACACCAAACCAGAAGCC	CATTCACCGAGGAAGCAGAA	50.0	50.0	58.3	57.9	497-549 bp	1.0	77.7