Hotspot

trnS-GCU-trnR-UCU

LSC · 7972-9810 · 1839 bp

Back to Poilannammia

Pi 0.0111
MarkerSeek score 57.80
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1839
variable_sites
20
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1920-1952 bp 1.0 70.7
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1919-1951 bp 1.0 70.7
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1927-1959 bp 1.0 68.8
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1926-1958 bp 1.0 68.8
trnS-GCU-trnR-UCU_p5 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1921-1953 bp 1.0 68.7