Hotspot

ycf1

SSC · 123989-129499 · 5511 bp

Back to Poikilospermum

Pi 0.00236

MarkerSeek score 39.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5511
variable_sites: 13
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACCAGCCCATCTCAATTGA	ACCAATCAAGTCGCTCCTTT	50.0	45.0	57.6	57.4	299 bp	1.0	72.6
ycf1_p2	AGACCAGCCCATCTCAATTG	ACCAATCAAGTCGCTCCTTT	50.0	45.0	57.6	57.4	300 bp	1.0	72.5
ycf1_p3	ACCAGCCCATCTCAATTGAA	ACCAATCAAGTCGCTCCTTT	45.0	45.0	57.1	57.4	298 bp	1.0	68.7
ycf1_p4	GACCAGCCCATCTCAATTGA	ACCATACCAATCAAGTCGCT	50.0	45.0	57.6	56.9	304 bp	1.0	68.5
ycf1_p5	AGACCAGCCCATCTCAATTG	ACCATACCAATCAAGTCGCT	50.0	45.0	57.6	56.9	305 bp	1.0	68.4