Hotspot

ndhF

SSC · 109818-112052 · 2235 bp

Back to Poikilospermum

Pi 0.00358
MarkerSeek score 59.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2235
variable_sites
8
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AAACATTTACGCCGCTTTCA ACCGATCCCTTTCATTTCAA 40.0 40.0 56.9 54.9 2341 bp 1.0 47.1
ndhF_p2 AACATTTACGCCGCTTTCAA AACCGATCCCTTTCATTTCA 40.0 40.0 56.9 54.9 2341 bp 1.0 47.1
ndhF_p3 AACATTTACGCCGCTTTCAA ACCGATCCCTTTCATTTCAA 40.0 40.0 56.9 54.9 2340 bp 1.0 47.1
ndhF_p4 ACATTTACGCCGCTTTCAAA AACCGATCCCTTTCATTTCA 40.0 40.0 56.9 54.9 2340 bp 1.0 47.1
ndhF_p5 ACATTTACGCCGCTTTCAAA ACCGATCCCTTTCATTTCAA 40.0 40.0 56.9 54.9 2339 bp 1.0 47.1