Hotspot

ndhA

SSC · 119734-122027 · 2294 bp

Back to Poikilospermum

Pi 0.00262

MarkerSeek score 58.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2294
variable_sites: 6
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GCCCGCCTGGTTTTATCAAT	47.6	50.0	58.1	58.9	2522 bp	1.0	64.5
ndhA_p2	TGAACCCAGTTACCATAGGGA	GCCCGCCTGGTTTTATCAAT	47.6	50.0	57.8	58.9	2525 bp	1.0	63.4
ndhA_p3	ACCCAGTTACCATAGGGAACA	GCCCGCCTGGTTTTATCAA	47.6	52.6	58.1	58.4	2522 bp	1.0	60.2
ndhA_p4	TGAACCCAGTTACCATAGGGA	GCCCGCCTGGTTTTATCAA	47.6	52.6	57.8	58.4	2525 bp	1.0	59.1
ndhA_p5	CCCAGTTACCATAGGGAACA	GCCCGCCTGGTTTTATCAAT	50.0	50.0	56.2	58.9	2521 bp	1.0	58.8