Hotspot

clpP

LSC · 70147-72144 · 1998 bp

Back to Poikilospermum

Pi 0.00251

MarkerSeek score 48.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1998
variable_sites: 5
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACTTGCGTAAAATCTCTCTT	ACGCTTTCACATCAAAGTGA	35.0	40.0	52.5	55.9	2067-2071 bp	1.0	40.4
clpP_p2	ACTTGCGTAAAATCTCTCTT	ACCCATTTTTACGCTTTCACA	35.0	38.1	52.5	56.6	2077-2081 bp	1.0	40.4
clpP_p3	ACTTGCGTAAAATCTCTCTT	AACCCATTTTTACGCTTTCACA	35.0	36.4	52.5	57.3	2078-2082 bp	1.0	40.4
clpP_p4	ACTTGCGTAAAATCTCTCTT	ACCCATTTTTACGCTTTCACAT	35.0	36.4	52.5	57.1	2077-2081 bp	1.0	40.4
clpP_p5	ACTTGCGTAAAATCTCTCTTA	ACGCTTTCACATCAAAGTGA	33.3	40.0	52.6	55.9	2067-2071 bp	1.0	40.4