Hotspot

trnN-GUU-trnR-ACG

IRa · 129885-130459 · 575 bp

Back to Poikilospermum

Pi 0.00696

MarkerSeek score 62.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 575
variable_sites: 4
pi: 0.00696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	757 bp	1.0	77.9
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	680 bp	1.0	77.5
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	667 bp	1.0	77.5
trnN-GUU-trnR-ACG_p4	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	794 bp	1.0	77.4
trnN-GUU-trnR-ACG_p5	ACCTCCTGTTGTTCCTCAGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	721 bp	1.0	77.2