Hotspot

psbE-petL

LSC · 64714-65907 · 1194 bp

Back to Poikilospermum

Pi 0.00168

MarkerSeek score 59.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 2
pi: 0.00168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCTGAGGTTATAGTCAACGC	45.0	47.6	58.0	57.8	1367-1369 bp	1.0	70.8
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCTGAGGTTATAGTCAACGCT	45.0	47.6	58.0	57.8	1366-1368 bp	1.0	70.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCTGAGGTTATAGTCAACGC	50.0	47.6	57.9	57.8	1356-1358 bp	1.0	70.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCTGAGGTTATAGTCAACGCT	50.0	47.6	57.9	57.8	1355-1357 bp	1.0	70.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GCTGAGGTTATAGTCAACGCT	50.0	47.6	57.8	57.8	1278-1280 bp	1.0	69.7