Hotspot

ycf3-trnS-GGA

LSC · 44424-45324 · 901 bp

Back to Poikilospermum

Pi 0.00333

MarkerSeek score 60.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 3
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CTCTTTCTCCCCCGAAGTTG	AGGTGAAGGGTACGGAAAGA	55.0	50.0	57.9	58.0	1102-1103 bp	1.0	79.3
ycf3-trnS-GGA_p2	CTTTCTCCCCCGAAGTTGTC	AGGTGAAGGGTACGGAAAGA	55.0	50.0	58.2	58.0	1100-1101 bp	1.0	78.5
ycf3-trnS-GGA_p3	CTCTTTCTCCCCCGAAGTTG	TGGTGAACTAGGTGAAGGGT	55.0	50.0	57.9	57.9	1111-1112 bp	1.0	78.5
ycf3-trnS-GGA_p4	CTCTTTCTCCCCCGAAGTTG	ACGGAAAGAGAGGGATTCGA	55.0	50.0	57.9	58.2	1091-1092 bp	1.0	78.2
ycf3-trnS-GGA_p5	AAGCGAATGCCTCTTTCTCC	AGGTGAAGGGTACGGAAAGA	50.0	50.0	58.3	58.0	1112-1113 bp	1.0	78.0