Hotspot

psbZ-trnG-GCC

LSC · 35871-36319 · 449 bp

Back to Poikilospermum

Pi 0.0112

MarkerSeek score 62.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 5
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGTATTTGCTTCTCCCGACG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	622 bp	1.0	80.4
psbZ-trnG-GCC_p2	TGTATTTGCTTCTCCCGACG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	623 bp	1.0	80.4
psbZ-trnG-GCC_p3	TGTATTTGCTTCTCCCGACG	CATCTTCTCCGTGGCAAAGA	50.0	50.0	58.0	57.9	611 bp	1.0	80.1
psbZ-trnG-GCC_p4	TGTATTTGCTTCTCCCGACG	CTTCTCCGTGGCAAAGAGAA	50.0	50.0	58.0	57.8	608 bp	1.0	79.5
psbZ-trnG-GCC_p5	TGTATTTGCTTCTCCCGACG	ATCTTCTCCGTGGCAAAGAG	50.0	50.0	58.0	57.6	610 bp	1.0	77.7