Hotspot

petN-psbM

LSC · 28923-29484 · 562 bp

Back to Poikilospermum

Pi 0.00713

MarkerSeek score 62.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 4
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGAGGACAAAACCCACATG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.1	57.6	747-748 bp	1.0	68.4
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	723-724 bp	1.0	68.1
petN-psbM_p3	GATCGAGGACAAAACCCACA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	749-750 bp	1.0	67.8
petN-psbM_p4	GAGGACAAAACCCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	745-746 bp	1.0	67.0
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	662-663 bp	1.0	66.5