Hotspot

trnK-UUU-rps16

LSC · 4256-4942 · 687 bp

Back to Poikilospermum

Pi 0.00437

MarkerSeek score 61.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 3
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.9	58.3	908-909 bp	1.0	77.3
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.9	58.3	907-908 bp	1.0	77.3
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	825-826 bp	1.0	76.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	824-825 bp	1.0	76.5
trnK-UUU-rps16_p5	CAGTCGCGGTCTTACAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	909-910 bp	1.0	76.1