Hotspot

trnH-GUG-psbA

LSC · 77-285 · 209 bp

Back to Poikilospermum

Pi 0.0478

MarkerSeek score 68.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 10
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACCGCGCTAATCTTGGTATG	55.0	50.0	58.1	58.1	329 bp	1.0	80.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	343 bp	1.0	80.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	342 bp	1.0	80.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	344 bp	1.0	80.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGCGCTAATCTTGGTATGGA	55.0	50.0	58.1	57.9	327 bp	1.0	80.2