Hotspot

cemA

Genome · 21815-23320 · 1506 bp

Pi 0.0321

MarkerSeek score 65.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 1506
variable_sites: 70
pi: 0.0321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AATACGACCGGTTGCTTCTC	GGCATTTATCCTCGGCGTAT	50.0	50.0	58.0	57.9	2103-2130 bp	1.0	84.4
cemA_p2	GCACATACAATACGACCGGT	GGCATTTATCCTCGGCGTAT	50.0	50.0	58.1	57.9	2111-2138 bp	1.0	83.8
cemA_p3	AATACGACCGGTTGCTTCTC	TGAATGGCTTTGGGTAGAGC	50.0	50.0	58.0	57.9	2491-2518 bp	0.666667	68.8
cemA_p4	GCACATACAATACGACCGGT	TGAATGGCTTTGGGTAGAGC	50.0	50.0	58.1	57.9	2499-2526 bp	0.666667	68.2
cemA_p5	CGGATATGCTTCGGAGATCG	GGCATTTATCCTCGGCGTAT	55.0	50.0	58.1	57.9	2079 bp	0.333333	51.8