Hotspot

rrn5-rrn4.5

Genome · 116678-116876 · 199 bp

Back to Pohlia

Pi 0.057
MarkerSeek score 81.50
Primer available yes
Label rrn5-rrn4.5

Feature Metadata

label_name
rrn5-rrn4.5
feature_type
igs
region
Genome
length_bp
199
variable_sites
17
pi
0.057
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-rrn4.5_p1 TGTGGTTCCTCTACGCCTAA CAGCTGAGGCATCCTAACAG 50.0 55.0 58.1 58.0 263-267 bp 1.0 85.4
rrn5-rrn4.5_p2 TGTGGTTCCTCTACGCCTAA GCTGAGGCATCCTAACAGAC 50.0 55.0 58.1 58.1 261-265 bp 1.0 85.4
rrn5-rrn4.5_p3 CCCTACAGTATTGTCACCGC CAGCTGAGGCATCCTAACAG 55.0 55.0 58.1 58.0 318-322 bp 1.0 84.3
rrn5-rrn4.5_p4 CCCTACAGTATTGTCACCGC GCTGAGGCATCCTAACAGAC 55.0 55.0 58.1 58.1 316-320 bp 1.0 84.3
rrn5-rrn4.5_p5 CCCTACAGTATTGTCACCGC CCAAGATAAGGTCACGGCAA 55.0 50.0 58.1 57.9 391-395 bp 1.0 82.9