Hotspot

ndhE-ndhI

Genome · 102756-103515 · 760 bp

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Pi 0.0487
MarkerSeek score 75.90
Primer available yes
Label ndhE-ndhI

Feature Metadata

label_name
ndhE-ndhI
feature_type
igs
region
Genome
length_bp
760
variable_sites
54
pi
0.0487
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhI_p1 ACATCCATCCCAAGGTACCA AGCACGGTATATTGGCCAAG 50.0 50.0 58.0 58.0 1814-1823 bp 1.0 84.5
ndhE-ndhI_p2 TGCTCGAACCATATTCCGAC AGCACGGTATATTGGCCAAG 50.0 50.0 57.8 58.0 1338-1346 bp 1.0 83.4
ndhE-ndhI_p3 ACATCTGTAGGACACGCTCT AGCACGGTATATTGGCCAAG 50.0 50.0 58.2 58.0 1787-1795 bp 0.666667 68.5
ndhE-ndhI_p4 ACACGCTCTTACACATTGGG AGCACGGTATATTGGCCAAG 50.0 50.0 58.2 58.0 1784 bp 0.333333 51.5
ndhE-ndhI_p5 CACGCTCTTACACATTGGGT AGCACGGTATATTGGCCAAG 50.0 50.0 58.2 58.0 1783 bp 0.333333 51.5