Hotspot

trnR-UCU-atpA

Genome · 64037-64378 · 342 bp

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Pi 0.11
MarkerSeek score 78.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
Genome
length_bp
342
variable_sites
55
pi
0.11
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ACCCTTAGCCTTCCAAGCTA AGAGGTCAGAGATTACGCGA 50.0 50.0 58.0 58.0 802-805 bp 1.0 87.4
trnR-UCU-atpA_p2 CGCCGACTATAACCCTTAGC AGAGGTCAGAGATTACGCGA 55.0 50.0 57.9 58.0 813-816 bp 1.0 87.0
trnR-UCU-atpA_p3 GCGCCGACTATAACCCTTAG AGAGGTCAGAGATTACGCGA 55.0 50.0 57.9 58.0 814-817 bp 1.0 87.0
trnR-UCU-atpA_p4 ACCCTTAGCCTTCCAAGCTA CCAGGTCGTGAAGCATATCC 50.0 55.0 58.0 58.1 1195-1198 bp 1.0 84.7
trnR-UCU-atpA_p5 ACCCTTAGCCTTCCAAGCTA GGTCGTGAAGCATATCCAGG 50.0 55.0 58.0 58.1 1192-1195 bp 1.0 84.7