Hotspot

trnK-UUU-chlB

Genome · 58635-59333 · 699 bp

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Pi 0.0573
MarkerSeek score 82.40
Primer available yes
Label trnK-UUU-chlB

Feature Metadata

label_name
trnK-UUU-chlB
feature_type
igs
region
Genome
length_bp
699
variable_sites
56
pi
0.0573
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-chlB_p1 AACGCTGTTGCCATACAAGT CGTTAGTTGCACGGGTACTT 45.0 50.0 58.4 58.2 1849-1867 bp 1.0 80.8
trnK-UUU-chlB_p2 ACGCTGTTGCCATACAAGTT CGTTAGTTGCACGGGTACTT 45.0 50.0 58.4 58.2 1848-1866 bp 1.0 80.8
trnK-UUU-chlB_p3 ACGCTGTTGCCATACAAGTT CCGTTGTTTTTGGTGATGCA 45.0 45.0 58.4 57.8 1918-1936 bp 1.0 80.6
trnK-UUU-chlB_p4 AACGCTGTTGCCATACAAGT GGAATTCGCGTTAGTTGCAC 45.0 50.0 58.4 58.1 1857-1875 bp 0.666667 67.6
trnK-UUU-chlB_p5 ACGCTGTTGCCATACAAGTT GGAATTCGCGTTAGTTGCAC 45.0 50.0 58.4 58.1 1856-1874 bp 0.666667 67.6