Hotspot

psbD-trnT-GGU

Genome · 45882-46510 · 629 bp

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Pi 0.0461
MarkerSeek score 80.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
629
variable_sites
43
pi
0.0461
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 ACGGTCTCTTCTTAGCCAGT GTGGTAGAGTAACGCCATGG 50.0 55.0 58.1 58.1 768-772 bp 1.0 83.5
psbD-trnT-GGU_p2 ACGGTCTCTTCTTAGCCAGT TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.1 58.1 771-775 bp 1.0 83.3
psbD-trnT-GGU_p3 ACGGTCTCTTCTTAGCCAGT GGCGTAAGTCATCGGTTCAA 50.0 50.0 58.1 58.3 745-749 bp 1.0 81.9
psbD-trnT-GGU_p4 ACGGTCTCTTCTTAGCCAGT TGGTAGAGTAACGCCATGGT 50.0 50.0 58.1 58.4 767-771 bp 1.0 80.5
psbD-trnT-GGU_p5 GCCAGTCATCCATGCTATCA GTGGTAGAGTAACGCCATGG 50.0 55.0 57.4 58.1 754-758 bp 1.0 79.7