Hotspot

cemA-ycf4

Genome · 23321-23439 · 119 bp

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Pi 0.0833
MarkerSeek score 78.10
Primer available yes
Label cemA-ycf4

Feature Metadata

label_name
cemA-ycf4
feature_type
igs
region
Genome
length_bp
119
variable_sites
13
pi
0.0833
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-ycf4_p1 ATTATGGCTTGCCAAGACCG GGCATTTATCCTCGGCGTAT 50.0 50.0 58.6 57.9 421-436 bp 1.0 81.5
cemA-ycf4_p2 ATTATGGCTTGCCAAGACCG TATTCGGTGCACTTGGGTTT 50.0 45.0 58.6 57.7 738-753 bp 1.0 78.2
cemA-ycf4_p3 ATTATGGCTTGCCAAGACCG TTATTCGGTGCACTTGGGTT 50.0 45.0 58.6 57.7 739-754 bp 1.0 78.1
cemA-ycf4_p4 ATTATGGCTTGCCAAGACCG AGAAGGCATTTATCCTCGGC 50.0 50.0 58.6 57.7 425-440 bp 0.666667 65.1
cemA-ycf4_p5 ATTATGGCTTGCCAAGACCG TGAATGGCTTTGGGTAGAGC 50.0 50.0 58.6 57.9 809-824 bp 0.666667 64.2