Hotspot

rpl2-rps19

Genome · 2147-2200 · 54 bp

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Pi 0.0741
MarkerSeek score 79.90
Primer available yes
Label rpl2-rps19

Feature Metadata

label_name
rpl2-rps19
feature_type
igs
region
Genome
length_bp
54
variable_sites
6
pi
0.0741
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rps19_p1 CGTCGTCGTAAAAATGGATA TGATCAGCTACAAAAGGGCC 40.0 50.0 53.6 57.9 114-117 bp 1.0 49.5
rpl2-rps19_p2 TGATCCTTTAATTTTACGTCGTCG TGATCAGCTACAAAAGGGCC 37.5 50.0 57.7 57.9 130-133 bp 1.0 49.3
rpl2-rps19_p3 CGTCGTCGTAAAAATGGATAA TGATCAGCTACAAAAGGGCC 38.1 50.0 54.5 57.9 114-117 bp 1.0 48.3
rpl2-rps19_p4 TCCTTTAATTTTACGTCGTCG TGATCAGCTACAAAAGGGCC 38.1 50.0 54.4 57.9 127-130 bp 1.0 47.2
rpl2-rps19_p5 TGATCCTTTAATTTTACGTCGTCG GATCAGCTACAAAAGGGCCT 37.5 50.0 57.7 57.6 129-132 bp 1.0 46.9