Hotspot

ycf1

SSC · 122345-127888 · 5544 bp

Back to Pogostemon

Pi 0.0229

MarkerSeek score 54.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 237
pi: 0.0229
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACAATTTCTGTTGATAATGGT	TGAACGGATCATATCGTAAAAGA	27.3	34.8	52.2	55.6	185 bp	1.0	46.5
ycf1_p2	ACAATTTCTGTTGATAATGGTT	TGAACGGATCATATCGTAAAAGA	27.3	34.8	52.2	55.6	184 bp	1.0	46.5
ycf1_p3	AACAATTTCTGTTGATAATGGTT	TGAACGGATCATATCGTAAAAGA	26.1	34.8	53.1	55.6	185 bp	1.0	46.5
ycf1_p4	ACAATTTCTGTTGATAATGGTTT	TGAACGGATCATATCGTAAAAGA	26.1	34.8	53.1	55.6	184 bp	1.0	46.5
ycf1_p5	ACAATTTCTGTTGATAATGGTT	TGAACGGATCATATCGTAAAAGAA	27.3	33.3	52.2	56.3	184 bp	1.0	46.5