Hotspot

ndhF

SSC · 109178-111412 · 2235 bp

Back to Pogostemon

Pi 0.0185

MarkerSeek score 55.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 78
pi: 0.0185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TAACTTGTCAAACCCGCGAA	50.0	45.0	58.0	58.0	2728-2741 bp	1.0	82.6
ndhF_p2	AAGAGGGATCCACCGAAGAA	TAACTTGTCAAACCCGCGAA	50.0	45.0	58.0	58.0	2720-2733 bp	1.0	82.6
ndhF_p3	ACAGGAACAAGAGGGATCCA	GAAGGGCATGGGAAAATTGC	50.0	50.0	58.0	58.0	2793-2796 bp	0.6	64.1
ndhF_p4	AAGAGGGATCCACCGAAGAA	GAAGGGCATGGGAAAATTGC	50.0	50.0	58.0	58.0	2785-2788 bp	0.6	64.1
ndhF_p5	AGGGCGGATCCAAACAAAAT	GAAGGGCATGGGAAAATTGC	45.0	50.0	58.1	58.0	2735-2738 bp	0.6	64.0