Hotspot

rps15-ycf1

SSC · 121945-122344 · 400 bp

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Pi 0.035

MarkerSeek score 68.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 400
variable_sites: 26
pi: 0.035
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	ATGGCAGTCGTTTCGGTATG	50.0	50.0	58.0	58.3	627-654 bp	1.0	84.4
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	ATGGCAGTCGTTTCGGTATG	45.0	50.0	58.0	58.3	623-650 bp	1.0	84.3
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	AATGGCAGTCGTTTCGGTAT	50.0	45.0	58.0	57.6	628-655 bp	1.0	84.0
rps15-ycf1_p4	CAGCAGTCGTTGACGTTTTC	ATAATGGCAGTCGTTTCGGT	50.0	45.0	58.0	57.6	630-657 bp	1.0	84.0
rps15-ycf1_p5	AACACCATGCATTGATGGGT	ATGGCAGTCGTTTCGGTATG	45.0	50.0	58.1	58.3	893-910 bp	1.0	82.7