Hotspot

ndhD-psaC

SSC · 115675-115766 · 92 bp

Back to Pogostemon

Pi 0.0913

MarkerSeek score 78.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 92
variable_sites: 14
pi: 0.0913
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCAAGGAAAATGGTTCGTGG	GACCGAAGACTGTGTTGGTT	50.0	50.0	57.9	58.1	243-250 bp	1.0	85.2
ndhD-psaC_p2	GCAAGGAAAATGGTTCGTGG	AAGGACCGAAGACTGTGTTG	50.0	50.0	57.9	57.8	246-253 bp	1.0	83.6
ndhD-psaC_p3	GCAAGGAAAATGGTTCGTGG	CAAGGACCGAAGACTGTGTT	50.0	50.0	57.9	57.8	247-254 bp	1.0	83.6
ndhD-psaC_p4	GCAAGGAAAATGGTTCGTGG	ACCGAAGACTGTGTTGGTTG	50.0	50.0	57.9	58.3	242-249 bp	1.0	83.0
ndhD-psaC_p5	GCAAGGAAAATGGTTCGTGG	CCGAAGACTGTGTTGGTTGT	50.0	50.0	57.9	58.3	241-248 bp	1.0	83.0