Hotspot

rpl32-trnL-UAG

SSC · 112090-112825 · 736 bp

Back to Pogostemon

Pi 0.0402

MarkerSeek score 77.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 57
pi: 0.0402
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCGCGGGTTTGACAAGTTA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1165-1184 bp	1.0	86.7
rpl32-trnL-UAG_p2	TTCGCGGGTTTGACAAGTTA	TGATCCACATAAAAGGGCCG	45.0	50.0	58.0	57.9	1751-1770 bp	1.0	85.4
rpl32-trnL-UAG_p3	GGGCAGCGTTAAAAGCATTT	TGATCCACATAAAAGGGCCG	45.0	50.0	57.9	57.9	1434-1465 bp	1.0	85.3
rpl32-trnL-UAG_p4	GCAATTTTCCCATGCCCTTC	TGATCCACATAAAAGGGCCG	50.0	50.0	58.0	57.9	1707-1715 bp	0.6	65.3
rpl32-trnL-UAG_p5	GCAATTTTCCCATGCCCTTC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1121-1129 bp	0.6	65.2