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psbT-psbN

LSC · 73767-73832 · 66 bp

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Pi 0.0485

MarkerSeek score 69.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 6
pi: 0.0485
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AAAACTGGGGGATCCGACTA	CTAGGAAGGCGACAGACCTA	50.0	55.0	58.0	57.9	1042-1060 bp	1.0	82.9
psbT-psbN_p2	AAAACTGGGGGATCCGACTA	CGCACACTCATATTCCGGAA	50.0	50.0	58.0	58.1	986-1004 bp	1.0	82.9
psbT-psbN_p3	GTAGACGGCGATGGAATTGT	CTAGGAAGGCGACAGACCTA	50.0	55.0	58.1	57.9	1419-1437 bp	1.0	82.1
psbT-psbN_p4	GTAGACGGCGATGGAATTGT	CGCACACTCATATTCCGGAA	50.0	50.0	58.1	58.1	1363-1381 bp	1.0	82.1
psbT-psbN_p5	ACACATTTGGCATGGTGCTA	CTAGGAAGGCGACAGACCTA	45.0	55.0	58.1	57.9	1144-1153 bp	0.6	63.5