Hotspot

petA-psbJ

LSC · 62906-63845 · 940 bp

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Pi 0.0289

MarkerSeek score 67.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 51
pi: 0.0289
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GTCTCTAGCGTGACCACTTG	50.0	55.0	58.0	58.0	1211-1257 bp	1.0	84.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ATTGGAGGGACACGGAGTAA	50.0	50.0	58.0	58.1	1182-1228 bp	1.0	84.3
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	TGGAGGGACACGGAGTAAAT	50.0	50.0	58.0	58.1	1180-1226 bp	1.0	84.3
petA-psbJ_p4	GCTCCTCCCGATCGTATTTC	GTCTCTAGCGTGACCACTTG	55.0	55.0	57.9	58.0	1701-1747 bp	1.0	83.2
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1746-1792 bp	1.0	83.0