Hotspot

trnM-CAU-atpE

LSC · 52060-52282 · 223 bp

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Pi 0.0448

MarkerSeek score 75.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 223
variable_sites: 22
pi: 0.0448
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGGATGGGTTCCTTTTGGTT	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.0	57.9	397-401 bp	1.0	83.2
trnM-CAU-atpE_p2	CGATGGGATGGGTTCCTTTT	GAGGCAAATCTAGCTCTCCG	50.0	55.0	57.9	57.9	401-405 bp	1.0	82.2
trnM-CAU-atpE_p3	ATGGGATGGGTTCCTTTTGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	57.7	57.9	399-403 bp	1.0	81.4
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	334-336 bp	1.0	81.0
trnM-CAU-atpE_p5	ATCGATGGGATGGGTTCCTT	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.5	57.9	403-407 bp	1.0	79.6