Hotspot

trnT-UGU-trnL-UAA

LSC · 46161-46841 · 681 bp

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Pi 0.0179

MarkerSeek score 68.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 23
pi: 0.0179
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	707-743 bp	1.0	83.4
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	739-775 bp	1.0	82.3
trnT-UGU-trnL-UAA_p3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	758-794 bp	1.0	82.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	701-737 bp	1.0	82.2
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	752-788 bp	1.0	80.9