Hotspot

trnS-GGA-rps4

LSC · 44849-45113 · 265 bp

Back to Pogostemon

Pi 0.0356

MarkerSeek score 68.40

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 17
pi: 0.0356
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGTAAGTGGGTCGGTTTGAA	50.0	45.0	58.1	56.7	370-372 bp	1.0	73.1
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	AGTAAGTGGGTCGGTTTGAAA	50.0	42.9	58.1	57.4	370-372 bp	1.0	71.0
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	AGTAAGTGGGTCGGTTTGAA	50.0	45.0	58.4	56.7	369-371 bp	1.0	70.8
trnS-GGA-rps4_p4	GGCTTTTGTTTACCGAGGGT	AGTAAGTGGGTCGGTTTGAAA	50.0	42.9	58.4	57.4	369-371 bp	1.0	68.7
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	GTAAGTGGGTCGGTTTGAAA	50.0	45.0	58.1	55.9	369-371 bp	1.0	66.8