Hotspot

ycf3-trnS-GGA

LSC · 43848-44761 · 914 bp

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Pi 0.0243

MarkerSeek score 73.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 40
pi: 0.0243
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1050-1086 bp	1.0	79.3
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1049-1085 bp	1.0	79.2
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1071-1107 bp	1.0	78.7
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	1070-1106 bp	1.0	78.7
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	1021-1057 bp	1.0	78.6