Hotspot

trnH-GUG-psbA

LSC · 77-286 · 210 bp

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Pi 0.0393

MarkerSeek score 71.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 15
pi: 0.0393
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTTCTATCGAAGCTCCAACA	55.0	45.0	58.1	54.9	249-261 bp	1.0	62.9
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CTTCTATCGAAGCTCCAACA	50.0	45.0	57.9	54.9	262-274 bp	1.0	62.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTTCTATCGAAGCTCCAACA	50.0	45.0	57.9	54.9	263-275 bp	1.0	62.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTTCTATCGAAGCTCCAACA	50.0	45.0	57.9	54.9	264-276 bp	1.0	62.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CTTCTATCGAAGCTCCAACAA	55.0	42.9	58.1	55.7	249-261 bp	1.0	61.4