Hotspot

rps4

LSC · 46368-46973 · 606 bp

Back to Pogonia

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TACTTCAGCGAAAGGAGTGC	TGCTTTGTGGATACTGTAGCT	50.0	42.9	57.9	57.0	743 bp	1.0	63.6
rps4_p2	TCAGCGAAAGGAGTGCAAAT	TGCTTTGTGGATACTGTAGCT	45.0	42.9	58.1	57.0	739 bp	1.0	63.4
rps4_p3	TACTTCAGCGAAAGGAGTGC	TGTGGATACTGTAGCTCAGAGT	50.0	45.5	57.9	58.0	738 bp	1.0	63.2
rps4_p4	TCAGCGAAAGGAGTGCAAAT	TGTGGATACTGTAGCTCAGAGT	45.0	45.5	58.1	58.0	734 bp	1.0	63.0
rps4_p5	TACTTCAGCGAAAGGAGTGC	GCTTTGTGGATACTGTAGCTCA	50.0	45.5	57.9	58.1	742 bp	1.0	62.4