Hotspot

rpoC2

LSC · 33775-37845 · 4071 bp

Back to Pogonia

Pi 0.000491

MarkerSeek score 54.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4071
variable_sites: 2
pi: 0.000491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GAACTCTTGGCACGTTGTTG	TTTCAATTGAAGTGCTTTAAGCA	50.0	30.4	57.9	55.9	299 bp	1.0	40.3
rpoC2_p2	AACATCAGGGAACTCTTGGC	TTTCAATTGAAGTGCTTTAAGCA	50.0	30.4	57.8	55.9	308 bp	1.0	40.3
rpoC2_p3	AACTCTTGGCACGTTGTTGA	TTTCAATTGAAGTGCTTTAAGCA	45.0	30.4	58.3	55.9	298 bp	1.0	40.3
rpoC2_p4	ACTCTTGGCACGTTGTTGAA	TTTCAATTGAAGTGCTTTAAGCA	45.0	30.4	58.3	55.9	297 bp	1.0	40.3
rpoC2_p5	CTTGGCACGTTGTTGAATCG	TTTCAATTGAAGTGCTTTAAGCA	50.0	30.4	58.3	55.9	294 bp	1.0	40.3