Hotspot

psbA

LSC · 120-1181 · 1062 bp

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Pi 0

MarkerSeek score 29.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTGGGGGATTGTTACCTACA	GTGCTTGGGAGTCCTTGAAA	47.6	50.0	58.1	58.0	1159 bp	1.0	71.2
psbA_p2	ACTGGGGGATTGTTACCTACA	TGAATACGTGTGCTTGGGAG	47.6	50.0	58.1	57.9	1168 bp	1.0	70.6
psbA_p3	ACTGGGGGATTGTTACCTACA	AATACGTGTGCTTGGGAGTC	47.6	50.0	58.1	57.9	1166 bp	1.0	70.6
psbA_p4	ACTGGGGGATTGTTACCTACA	GAATACGTGTGCTTGGGAGT	47.6	50.0	58.1	57.9	1167 bp	1.0	70.6
psbA_p5	ACTGGGGGATTGTTACCTACA	AGTGAATACGTGTGCTTGGG	47.6	50.0	58.1	58.2	1170 bp	1.0	69.9