Hotspot

ccsA

SSC · 122439-123371 · 933 bp

Back to Pogonia

Pi 0.00107

MarkerSeek score 59.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 933
variable_sites: 1
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTCTGTCAGACGATGTTTTT	CGAGTGGCGGCATACAAATA	40.0	50.0	53.9	58.4	1089 bp	1.0	43.9
ccsA_p2	GTCTGTCAGACGATGTTTTT	TGGCGGCATACAAATAGACA	40.0	45.0	53.9	57.3	1085 bp	1.0	41.7
ccsA_p3	TCAAACGTTTTTCAAAAACTCGT	CGAGTGGCGGCATACAAATA	30.4	50.0	56.6	58.4	1131 bp	1.0	41.6
ccsA_p4	GTCTGTCAGACGATGTTTTTATGT	CGAGTGGCGGCATACAAATA	37.5	50.0	57.5	58.4	1089 bp	1.0	41.0
ccsA_p5	ACGTTTTTCAAAAACTCGTACA	CGAGTGGCGGCATACAAATA	31.8	50.0	55.4	58.4	1127 bp	1.0	40.1