Hotspot

rpl32-ndhA

SSC · 124417-125517 · 1101 bp

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Pi 0

MarkerSeek score 61.00

Primer available yes

Label rpl32-ndhA

Feature Metadata

label_name: rpl32-ndhA
feature_type: igs
region: SSC
length_bp: 1101
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhA_p1	CACAATGCATGGGAACGAAA	GCGTGATTGGTTGGAACATG	45.0	50.0	57.3	58.0	1631-1657 bp	1.0	75.3
rpl32-ndhA_p2	CACAATGCATGGGAACGAAA	GCAATATCTCTCCGCGTGAT	45.0	50.0	57.3	57.9	1644-1670 bp	1.0	74.9
rpl32-ndhA_p3	CACAATGCATGGGAACGAAA	CAAGTCTTGTCCCCATTGCT	45.0	50.0	57.3	58.1	1772-1798 bp	1.0	74.7
rpl32-ndhA_p4	CACAATGCATGGGAACGAAA	TCAAGTCTTGTCCCCATTGC	45.0	50.0	57.3	58.1	1773-1799 bp	1.0	74.7
rpl32-ndhA_p5	CACAATGCATGGGAACGAAA	TGGGAAGTCCAATGGCAAAA	45.0	45.0	57.3	57.9	2195-2221 bp	1.0	74.4