Hotspot

rpl20-rps12

LSC · 70861-71673 · 813 bp

Back to Pogonia

Pi 0.00123

MarkerSeek score 59.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 1
pi: 0.00123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATGTATCCTCGT	AATCTCCTGCTCTTCGAGGA	50.0	50.0	57.4	57.8	903 bp	1.0	74.4
rpl20-rps12_p2	TCCGAGCTATGTATCCTCGT	ACGAAATCTCCTGCTCTTCG	50.0	50.0	57.4	57.7	907 bp	1.0	73.3
rpl20-rps12_p3	TCCGAGCTATGTATCCTCGT	CGCCATGCCAACCATTAAAC	50.0	50.0	57.4	58.4	965 bp	1.0	72.7
rpl20-rps12_p4	TCCGAGCTATGTATCCTCGT	TGTCACGAAATCTCCTGCTC	50.0	50.0	57.4	57.6	911 bp	1.0	72.6
rpl20-rps12_p5	TCCGAGCTATGTATCCTCGT	ATCTCCTGCTCTTCGAGGAT	50.0	50.0	57.4	57.6	902 bp	1.0	72.5