Hotspot

clpP

Genome · 74281-75069 · 789 bp

Back to Podocarpus

Pi 0.0326

MarkerSeek score 74.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 789
variable_sites: 64
pi: 0.0326
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGGGTGTTGAAAGACTTGGC	CAGCGGTAATGTGGAAAGGA	50.0	50.0	58.3	57.9	1268-1577 bp	1.0	88.7
clpP_p2	AGGGTGTTGAAAGACTTGGC	AGCCTTTTTCTTGCCCAAGA	50.0	45.0	58.3	57.9	1368-1677 bp	1.0	88.6
clpP_p3	AGGGTGTTGAAAGACTTGGC	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.3	58.1	979-991 bp	0.833333	78.3
clpP_p4	AGGGTGTTGAAAGACTTGGC	TAACCACGCGAATACCAAGG	50.0	50.0	58.3	58.0	1503-1514 bp	0.333333	51.1
clpP_p5	AGGGTGTTGAAAGACTTGGC	GCGAATACCAAGGGCCTAAA	50.0	50.0	58.3	58.0	1496-1507 bp	0.333333	50.8