Hotspot

trnK-UUU-chlB

Genome · 128380-129946 · 1567 bp

Back to Podocarpus

Pi 0.0724

MarkerSeek score 89.50

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 1567
variable_sites: 285
pi: 0.0724
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	AGTCGCACTTAAAAGCCGAG	GCTAAAGAAGCGTTTGGTACC	50.0	47.6	58.6	57.5	1636-2047 bp	1.0	75.7
trnK-UUU-chlB_p2	TAGTCGCACTTAAAAGCCGA	GCTAAAGAAGCGTTTGGTACC	45.0	47.6	57.3	57.5	1637-2048 bp	1.0	74.4
trnK-UUU-chlB_p3	TCGCACTTAAAAGCCGAGTA	GCTAAAGAAGCGTTTGGTACC	45.0	47.6	57.3	57.5	1634-2045 bp	1.0	74.4
trnK-UUU-chlB_p4	CGCACTTAAAAGCCGAGTAC	GCTAAAGAAGCGTTTGGTACC	50.0	47.6	57.2	57.5	1633-2044 bp	1.0	74.0
trnK-UUU-chlB_p5	GTCGCACTTAAAAGCCGAGT	GCTAAAGAAGCGTTTGGTACC	50.0	47.6	58.9	57.5	1635-2046 bp	1.0	73.4