Hotspot

chlL-trnH-GUG

Genome · 122894-123635 · 742 bp

Back to Podocarpus

Pi 0.0287

MarkerSeek score 72.90

Primer available yes

Label chlL-trnH-GUG

Feature Metadata

label_name: chlL-trnH-GUG
feature_type: igs
region: Genome
length_bp: 742
variable_sites: 54
pi: 0.0287
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnH-GUG_p1	CCGATTCCGCCTTTTCCATA	CAAGTGGTTAAGGCAGTGGA	50.0	50.0	58.0	57.7	839-857 bp	1.0	83.8
chlL-trnH-GUG_p2	GGATTTACCGATTCCGCCTT	CAAGTGGTTAAGGCAGTGGA	50.0	50.0	58.0	57.7	846-864 bp	1.0	83.7
chlL-trnH-GUG_p3	ACGTCTGGCTAGAGCAATTG	CAAGTGGTTAAGGCAGTGGA	50.0	50.0	58.0	57.7	885-903 bp	1.0	83.6
chlL-trnH-GUG_p4	CACGTCTGGCTAGAGCAATT	CAAGTGGTTAAGGCAGTGGA	50.0	50.0	58.0	57.7	886-904 bp	1.0	83.6
chlL-trnH-GUG_p5	AACTGGTCGTGGATTTACCG	CAAGTGGTTAAGGCAGTGGA	50.0	50.0	57.9	57.7	856-874 bp	1.0	83.2