Hotspot

trnV-GAC-rps12

Genome · 107336-108265 · 930 bp

Back to Podocarpus

Pi 0.0173
MarkerSeek score 58.60
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
930
variable_sites
43
pi
0.0173
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 TCGTTGAACCGATGACCATC TCGAACCTGTTCCGAAAAGG 50.0 50.0 58.0 58.1 2057-2123 bp 0.666667 68.2
trnV-GAC-rps12_p2 TCGTTGAACCGATGACCATC TTTGACTCGAACCTGTTCCG 50.0 50.0 58.0 58.1 2063-2129 bp 0.666667 68.1
trnV-GAC-rps12_p3 TCGTTGAACCGATGACCATC GACTCGAACCTGTTCCGAAA 50.0 50.0 58.0 57.9 2060-2126 bp 0.666667 68.1
trnV-GAC-rps12_p4 TCGTTGAACCGATGACCATC CACTAGAACGCCCTTGTTGA 50.0 50.0 58.0 57.8 1781-1848 bp 0.666667 68.1
trnV-GAC-rps12_p5 TCGTTGAACCGATGACCATC ACTAGAACGCCCTTGTTGAC 50.0 50.0 58.0 57.8 1780-1847 bp 0.666667 68.1