Hotspot

trnI-GAU-rrn16

Genome · 103628-104257 · 630 bp

Back to Podocarpus

Pi 0.0338

MarkerSeek score 84.50

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: Genome
length_bp: 630
variable_sites: 57
pi: 0.0338
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	722-738 bp	1.0	83.4
trnI-GAU-rrn16_p2	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	57.8	57.7	773-789 bp	1.0	83.1
trnI-GAU-rrn16_p3	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	744-760 bp	1.0	83.1
trnI-GAU-rrn16_p4	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	57.8	58.3	795-811 bp	1.0	82.8
trnI-GAU-rrn16_p5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	50.0	50.0	57.8	57.6	782-798 bp	1.0	82.5