Hotspot

trnN-GUU-trnR-ACG

Genome · 97633-97717 · 85 bp

Back to Podocarpus

Pi 0.0494

MarkerSeek score 77.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 85
variable_sites: 11
pi: 0.0494
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	GGCTGTTAACCGATTAGTCGT	GTTCAAATCCCTCCTGGCTC	47.6	55.0	58.1	58.2	146-151 bp	0.833333	71.0
trnN-GUU-trnR-ACG_p2	GGCTGTTAACCGATTAGTCGT	AGAGGATTAGAGCACGTGGT	47.6	50.0	58.1	58.1	186-191 bp	0.833333	70.0
trnN-GUU-trnR-ACG_p3	GGCTGTTAACCGATTAGTCGT	CAGAGGATTAGAGCACGTGG	47.6	55.0	58.1	57.8	187-192 bp	0.833333	69.4
trnN-GUU-trnR-ACG_p4	GGCTGTTAACCGATTAGTCGT	GCTGAGAGCGGATCAAGTAG	47.6	55.0	58.1	57.9	224-229 bp	0.833333	69.0
trnN-GUU-trnR-ACG_p5	GGCTGTTAACCGATTAGTCGT	GAGCGGATCAAGTAGAGACG	47.6	55.0	58.1	57.6	219-224 bp	0.833333	67.0