Hotspot

rpl32-ndhF

Genome · 94065-94807 · 743 bp

Back to Podocarpus

Pi 0.0293

MarkerSeek score 73.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 743
variable_sites: 58
pi: 0.0293
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CTTTTACTGCGGCTCGGTAT	CCTATTGCCGCAGAAGCTAA	50.0	50.0	58.1	58.0	896-902 bp	1.0	84.9
rpl32-ndhF_p2	CGGTATGCTTTTCCCTTCCA	CCTATTGCCGCAGAAGCTAA	50.0	50.0	57.9	58.0	882-888 bp	1.0	84.5
rpl32-ndhF_p3	GCTCGGTATGCTTTTCCCTT	CCTATTGCCGCAGAAGCTAA	50.0	50.0	58.2	58.0	885-891 bp	1.0	83.5
rpl32-ndhF_p4	CTTTTACTGCGGCTCGGTAT	TAAAAGCGGAAGGAAAGGCA	50.0	45.0	58.1	57.7	879-885 bp	1.0	83.1
rpl32-ndhF_p5	TTTTACTGCGGCTCGGTATG	CCTATTGCCGCAGAAGCTAA	50.0	50.0	58.4	58.0	895-901 bp	1.0	82.6