Hotspot

ndhI-ndhG

Genome · 88228-88534 · 307 bp

Back to Podocarpus

Pi 0.0638

MarkerSeek score 73.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: Genome
length_bp: 307
variable_sites: 55
pi: 0.0638
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	CAACAAGCAATACAGGCTGC	TGAGACCTAGCGTTAGAGGG	50.0	55.0	58.0	57.9	851-853 bp	1.0	87.5
ndhI-ndhG_p2	CAACAAGCAATACAGGCTGC	TGCTATCTCCTACGGTCCAG	50.0	55.0	58.0	58.0	1088-1090 bp	1.0	86.3
ndhI-ndhG_p3	CCTATCGCTTTGGGCAATCT	TGCTATCTCCTACGGTCCAG	50.0	55.0	58.0	58.0	1295-1297 bp	1.0	85.6
ndhI-ndhG_p4	CACGGCGACTGGGTTTATAA	TGCTATCTCCTACGGTCCAG	50.0	55.0	58.0	58.0	1116 bp	0.833333	74.5
ndhI-ndhG_p5	CACGGCGACTGGGTTTATAA	TGAGACCTAGCGTTAGAGGG	50.0	55.0	58.0	57.9	879 bp	0.833333	74.3