Hotspot

clpP-ycf1

Genome · 75070-75608 · 539 bp

Back to Podocarpus

Pi 0.0448

MarkerSeek score 77.30

Primer available yes

Label clpP-ycf1

Feature Metadata

label_name: clpP-ycf1
feature_type: igs
region: Genome
length_bp: 539
variable_sites: 53
pi: 0.0448
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-ycf1_p1	CAACCCGCTAGTACTTGGAC	GTGCAGCGATAAAAAGCGTT	55.0	45.0	58.0	58.0	998-1295 bp	1.0	90.5
clpP-ycf1_p2	CAACCCGCTAGTACTTGGAC	CAGCGATAAAAAGCGTTGCA	55.0	45.0	58.0	58.0	995-1292 bp	1.0	90.5
clpP-ycf1_p3	CAACCCGCTAGTACTTGGAC	TAACCACGCGAATACCAAGG	55.0	50.0	58.0	58.0	989-1000 bp	0.333333	53.7
clpP-ycf1_p4	AGAAAACGGAAACAGGGTCC	TAACCACGCGAATACCAAGG	50.0	50.0	58.0	58.0	749-760 bp	0.333333	53.7
clpP-ycf1_p5	GAAAACGGAAACAGGGTCCT	TAACCACGCGAATACCAAGG	50.0	50.0	58.0	58.0	748-759 bp	0.333333	53.7