Hotspot

psbT-psbB

Genome · 71781-71974 · 194 bp

Back to Podocarpus

Pi 0.0479

MarkerSeek score 74.50

Primer available yes

Label psbT-psbB

Feature Metadata

label_name: psbT-psbB
feature_type: igs
region: Genome
length_bp: 194
variable_sites: 25
pi: 0.0479
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbB_p1	CAATGGAGTAGTGCCCCATC	GGGGATGGAATAGCTGTTGG	55.0	55.0	58.0	58.0	1244-1256 bp	1.0	83.3
psbT-psbB_p2	CATCAATGGAGTAGTGCCCC	GGGGATGGAATAGCTGTTGG	55.0	55.0	58.0	58.0	1247-1259 bp	1.0	83.3
psbT-psbB_p3	CGAGCGGATGTTCTGGTTTA	GGGGATGGAATAGCTGTTGG	50.0	55.0	58.0	58.0	1367-1379 bp	1.0	83.1
psbT-psbB_p4	GCACGAGGACTAGTGGAAAG	GGGGATGGAATAGCTGTTGG	55.0	55.0	58.0	58.0	914-923 bp	0.833333	76.7
psbT-psbB_p5	GCACGAGGACTAGTGGAAAG	TGTACGTCGTATGCCTACCT	55.0	50.0	58.0	58.0	846-855 bp	0.833333	76.6